Reactivity of fungal CMH with the anti-CMH MAb.

<p>(A) ELISA analysis of the binding of Mabs to <i>S. apiospermum</i> CMH. The amount of antibody bound to CMHs was determined by incubation with a rabbit anti-mouse IgG. CMH from <i>S. apiospermum</i> (closed circle); CMH from <i>A. fumigatus</i> (closed square); CMH from <i>S. apiospermum</i> + unrelated antibody (open circle); negative control (open square). Inset: HPTLC of <i>S. apiospermum</i> CMH (spots a,b,c), which was developed in CHCl3: MeOH: 2 M NH4OH (40:10:1 v/v). Lane 1: stained with orcinol/ H2SO4; Lane 2: immunostaining with the anti-CMH MAb. Indirect immunofluorescence analysis of conidial (B and C) and mycelial (D and E) forms of <i>S. apiospermum</i> using the anti-CMH MAb. B and D: phase contrast; C and E: fluorescence signal.