TIF-IA KD-induced cell differentiation of mouse HSCs in ex vivo culture.

<p>(A) Scheme showing the experimental procedure used for the HSC ex vivo culture system. The HSCs were purified from 8- to 12-week-old wild type mice. The purified HSCs were transduced with a lentivirus that expressed a shRNA against TIF-IA and cultured in media containing SCF and TPO. On days 5, 7, 10, and 12 after lentivirus transduction, myeloid differentiation was analyzed by flow cytometry. (B, C) TIF-IA KD promoted the myeloid differentiation of HSCs in culture. Anti-Mac-1 and anti-Gr-1 were used as myeloid cell markers. (B) The upper and lower panels show the results for the shControl and shTIF-IA cultures, respectively. Each panel shows the flow cytometric profiles of GFP⁺ transduced cells. (C) The percentages of lineage⁻ cells (Mac-1⁻Gr-1⁻) and myeloid cells (Mac1⁺ single positive and Mac-1⁺Gr-1⁺) among the GFP⁺ cells are shown as bar graphs. Values are expressed as the mean ± S.D., <i>n</i> = 3. *<i>P</i><0.05.