Prophylactic rflagellin administration reduced liver mCMV load in WT B6 mice.

<p>WT B6 and TLR5 KO B6 mice were given 25 µg rflagellin or 0.2 ml PBS i.p 48 hours before a sub-lethal dose (1×10⁵ pfu/mouse i.p) of mCMV. Mice were sacrificed on day 3 and 10 after mCMV infection and viral load per liver was determined as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0096165#s2" target="_blank">Materials and Methods</a>. A. Virus titer in livers of rflagellin- and PBS-treated WT B6 mice. B. Virus titer in livers of rflagellin- and PBS-treated TLR5 KO B6 mice. The data are the representative of three similar experiments using 5 mice per group at each time point. C. WT B6 mice were given 25 µg rflagellin/mouse (rFlagellin 48 Hrs, closed circle) or 0.2 ml PBS (open circle) i.p 48 hours before or at the same time as (rFlagellin 0 Hr, closed triangle) a sub-lethal mCMV infection (1×10⁵ pfu/mouse i.p). Weights of individual mice were measured on day 0, 1, 2, 3, 4 and 8 days after mCMV infection. Percent weight changes per group of experimental mice are presented. D. Mice receiving rflagellin (rFlagellin 0 Hr) during mCMV infection became dehydrated and hunched and were sacrificed on day 3 after mCMV infection and liver viral load was determined. The data are the representative of two similar experiments using 5 mice per group. The symbols “*” and “**”represent the <i>p</i> values<0.05 and <0.005, respectively, Students <i>t</i>-Test.