rFlagellin increased NK cell lytic activity in the presence and absence of mCMV infection.

<p>Splenocytes were harvested on days 0 and 3 after mCMV infection and NK cell lytic activity was measured using standard 4-hour ⁵¹Cr-release assay by Yac-1 target cells as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0096165#s2" target="_blank">Materials and Methods</a>. A and B. The % cell lytic activity of NK cells of rflagellin- and PBS-treated WT B6 mice on days 0 and 3 after infection. C and D. The % cell lytic activity by NK cells harvested from rflagellin- and PBS-treated TLR5 KO B6 mice on day 0 and 3 post infection. E. Kinetics of NK cells lytic activity from the splenocytes harvested from rflagellin and PBS-treated WT B6 mice on day 0, 1, 2, 3 and 8 after mCMV infection. The data shown in A–D are representative of three independent experiments and data shown in E are from one experiment. 5 mice were used per group per time point. The “*” indicates p value<0.05, Student's T-test.