G₁ cell cycle arrest by KIOM-C.

<p>(<b>A</b>) HT1080 cells were treated with 1000 µg/ml KIOM-C for 12 and 24 h, fixed with ice-cold 70% ethanol, stained with PI, and evaluated for cell cycle distribution using flow cytometry. Data represent the means ± SD of two independent experiments. * <i>p</i><0.01 vs. untreated control. (<b>B</b>) The expression of cell cycle-related proteins was examined by Western blotting. Band intensities relative to those of untreated “control” cells were determined after normalizing to tubulin expression and are expressed as the means ± SD of two independent experiments.