DGKα is necessary for SDF-1α-induced cell invasion.

<p>MDA-MB-231 cells were infected with lentiviral vectors expressing an inducible shRNA against DGKα (shRNA-DGKα1) or an inducible control shRNA (shRNA-CTRL). Parental and infected cells were treated with 1 µg/ml doxycycline for 72 hours to promote shRNA transcription. A) 50,000 cells were plated on matrigel invasion chamber and incubated for 24 hours in presence or in absence of SDF-1α (100 ng/ml). Histogram reports mean ± SE of fold over control values from 3 independent experiments with *t-test p<0.05, **t-test p<0.01. B) The efficiency of DGKα down–regulation by shRNA was verified by quantitative RT-PCR. **t-test p<0.01. A) Cells were lysed and the efficiency of DGKα down–regulation by shRNA was verified by western blot, tubulin was used as a loading control.