<i>Lepre1^H662A/H662A</i> fibroblast procollagen secretion rate and collagen modification is normal.

<p>Analysis of procollagen secretion by the collagen pulse-chase assay suggests that the procollagen secreted from <i>Lepre1^H662A/H662A</i>fibroblasts is similar to <i>Lepre1^+/+</i> fibroblasts (A, B). Additionally, there does not appear to be a decrease in the amount of procollagen secreted from the <i>Lepre1^H662A/H662A</i> fibroblasts in comparison to<i>Lepre1^+/+</i> fibroblasts (A, B). These findings are in contrast to that of the <i>Crtap^−/−</i> fibroblasts, which have an increase in the rate of procollagen secretion (A). Collagen modification was assessed using the collagen steady-state assay. We observed no difference in the migration pattern of procollagen and collagen isolated from <i>Lepre1^+/+</i>(+/+) and <i>Lepre1^H662A/H662A</i> (H662A/H662A) fibroblasts (C). These assays were repeated three times.