Schematic diagrams and quantitative analysis of the designed cassettes hybridization with biochip.

<p>The arrangement of the probes on the biochips (panels A, B, C, and D) was as follows: a1, a2 – CT1; a3, a4 – HSV; a5 – RV; b1, b2 – CT2; b3, b4 – TOXO1; b5 – RV; c1, c2 – CT3; c3, c4 – TOXO2; d1, d2 – CMV1; d3, d4 – TOXO3; e1, e2 – CMV2; e3, e4 –TOXO4; e5 – bacterial DNA (negative control); c5, d5 were left empty to give background signals. White circles represent gene probes that hybridized with the respective targets on the cassette; grey circles represent gene probes that showed no hybridization and background. Error bars in the histograms are from three replicates and present the results of the Student's <i>t</i>-Tests procedure (*p<0.05). Panel (A) Cassette N1 shows the hybridization signals for three <i>Chlamydia trachomatis</i> probes (probes CT1 and CT2), and for a cryptic plasmid sequence (probe CT3). Panel (B) shows the hybridization signals for the probes CMV1 and CMV2 for <i>Cytomegalovirus</i>, the probe HSV for <i>Herpes simplex virus</i> (HSV), the TOXO1 probe for <i>Toxoplasma gondii</i> (TOXO1), and the RV probe for <i>Rubela virus</i> (RV) with cassettes N2 (shaded grey in the histogram) and cassette N3 (shaded black in the histogram). Panel (C) shows the hybridization patterns for the probes TOXO1, TOXO2, TOXO3 and TOXO4 for <i>Toxoplasma gondii</i> with cassette N4, revealing that the TOXO1 probe was unsuitable for inclusion in the TORCH biochip. Panel (D) shows the hybridization signals for probes CT1, CMV2, HSV, RV, and TOXO2 with cassette N5.