Inhibitory effects of panepoxydone on the proliferation of human breast cancer cells.

<p>MCF-7, MDA-MB-231, MDA-MB-468 and MDA-MB-453 breast cancer cells were grown in 96 well microtitre plates (5000 cells/well) and treated with increasing concentrations of PP or with DMSO (0.2% vehicle control) and analyzed by CellTiter Glow assay. (<b>A</b>) Time-dependent anti-proliferative effect of PP on breast cancer cells. Data are presented as the means ± SD (n = 2). (<b>B</b>) Dose-dependent anti-proliferative effect of PP on breast cancer cells after 72 hrs. Data are presented as the means ± SD (n = 4). PP inhibited cell viability in a dose-dependent manner for all the cell types suggesting anti-cancer activity of PP. (<b>C</b>) Morphological changes of breast cancer cells after treatment with PP. Based on the IC₅₀ value, 3 doses were selected for subsequent experiments: D1 (half of IC₅₀, D2 (IC₅₀₎ and D3 (2×IC₅₀). Cells (1.5×10⁴ cells/well) were seeded in 6-well plates and incubated with increasing concentrations of PP or DMSO (0.2% vehicle control) for 24 hrs. Morphological changes were observed under the inverted phase-contrast microscope and photographed. Representative micrographs are from one of the random fields of view (magnification 200X) of cells.