<p>(A) Semi-log plot of the V<sub>i</sub> concentration dependence of the trapped enzyme fraction with 1000 µM ATP (blue symbols) or 1000 µM ADP (red symbols), from the evaluation of with and , respectively. (B) Semi-log plot of the nucleotide dependence of the fraction of free enzyme, with 200 µM V<sub>i</sub> and either ATP (blue symbols) or ADP (red symbols), evaluating with and , respectively. Lines are the best fits to the Hill equation with <i>n</i> = 1. Values of <b>k</b> are given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0098804#pone-0098804-t002" target="_blank"><b>Table 2</b></a>.</p
Double mixing experiments using a three-syringe stopped-flow apparatus have given values of the seco...
<p>(A) ATP substrate dependent specific activity. Experiments were performed at 25 μM dThd and kinet...
<p><b>A</b>) Rate constants for CD39L2 in the presence of different concentrations of ATP. The react...
<p>Plot of the P<sub>i</sub> concentration dependence of the trapped enzyme fraction with 100 µM V<s...
<p>Semi-log plots of the steady-state ATP dependence of the normalized concentrations of (i) one-nuc...
<p>ATP dependence of trapping. Semi-log plot of the ATP concentration dependence of the untrapped en...
<p>(A) Transient behavior of ATPase activity (red) and the fraction of trapped enzyme (blue), evalua...
<p>Initial rates of phosphate release (<i>vi</i>) were measured in reaction mixtures containing two ...
<p>How ADP affects the ATP response was determined by measuring the fluorescence as a function of AT...
<p>Enzyme activity was determined at a fixed concentration of NAD (2 mM for IMPDHbt and 6 mM for IMP...
<p>Nitrogen atoms are rendered in blue, oxygen in red, and phosphorus in orange. Protein carbon atom...
The interaction of the protein product of the H−ras oncogene with a series of nucleoside di− and tri...
<p>Time-averaged number of interactions (N<sub>int</sub>) of each of the five positively charged res...
<p>Enzyme activity was determined at a fixed concentration of IMP (1 mM for IMPDHbt and 2 mM for IMP...
<p>(A) Catalytic potential plots for each of the enzyme modules as a function of the energy charge. ...
Double mixing experiments using a three-syringe stopped-flow apparatus have given values of the seco...
<p>(A) ATP substrate dependent specific activity. Experiments were performed at 25 μM dThd and kinet...
<p><b>A</b>) Rate constants for CD39L2 in the presence of different concentrations of ATP. The react...
<p>Plot of the P<sub>i</sub> concentration dependence of the trapped enzyme fraction with 100 µM V<s...
<p>Semi-log plots of the steady-state ATP dependence of the normalized concentrations of (i) one-nuc...
<p>ATP dependence of trapping. Semi-log plot of the ATP concentration dependence of the untrapped en...
<p>(A) Transient behavior of ATPase activity (red) and the fraction of trapped enzyme (blue), evalua...
<p>Initial rates of phosphate release (<i>vi</i>) were measured in reaction mixtures containing two ...
<p>How ADP affects the ATP response was determined by measuring the fluorescence as a function of AT...
<p>Enzyme activity was determined at a fixed concentration of NAD (2 mM for IMPDHbt and 6 mM for IMP...
<p>Nitrogen atoms are rendered in blue, oxygen in red, and phosphorus in orange. Protein carbon atom...
The interaction of the protein product of the H−ras oncogene with a series of nucleoside di− and tri...
<p>Time-averaged number of interactions (N<sub>int</sub>) of each of the five positively charged res...
<p>Enzyme activity was determined at a fixed concentration of IMP (1 mM for IMPDHbt and 2 mM for IMP...
<p>(A) Catalytic potential plots for each of the enzyme modules as a function of the energy charge. ...
Double mixing experiments using a three-syringe stopped-flow apparatus have given values of the seco...
<p>(A) ATP substrate dependent specific activity. Experiments were performed at 25 μM dThd and kinet...
<p><b>A</b>) Rate constants for CD39L2 in the presence of different concentrations of ATP. The react...