Real-Time PCR verification of Affymetrix miRNA microarray data.

<p>A. Expression of mature miRNAs. The graph demonstrates mean of log₂ fold change (VPA vs. solvent control) ± SEM for six upregulated and five downregulated miRNAs in four independent differentiation processes. <i>mir-9</i> expression was not changed significantly upon 16 days of VPA treatment. (n = 4, t-test, *p<0.05, **p<0.01, ***p<0.001). B. Expression of primary miRNA transcripts in neural-differentiated mES cells at day 16 of differentiation under VPA exposure. The graph demonstrates mean of log₂ fold change from three independent experiments (VPA vs. solvent control) ± SEM. (n = 3, t-test, *p<0.05, **p<0.01, ***p<0.001).