Proliferative cells can be isolated from adult human white matter.

<p><b>A</b>. Diagram depicting the different procedures followed in the present work for the isolation of proliferative cells from human white matter. <b>B</b>. The cell isolation protocol without (w/o) the sucrose centrifugation step gives rise to a monolayer culture, which could be expanded more than seven passages. However, only 28.57% of samples generated viable cultures with this procedure (<b>B</b>). Therefore, we added a final sucrose centrifugation, which allowed the obtaining of sphere-like colonies lightly adhered to the culture plate from all samples processed (<b>D</b>). These cells generated an adherent culture that could be expanded independently for more than five passages (<b>E</b>). When the initial spheres were passaged and reseeded at a cell density of 3000 cells/cm² or higher, new spheres were obtained (<b>C</b>), which, in turn, generated a new monolayer culture. These spheres could be passaged up to tertiary spheres, similarly to other proliferative cells isolated from adult human white matter. The scale bars represent 50 µm.