Recovery of mCx-I activity by incubation with anti-IL-1β blocking antibody or with IL-1β receptor antagonist (IL1RN).

<p>S9 and IB3-1 cells preincubated for 24 h in serum free media were additionally incubated for 24 h (in the presence of a CFTR-stimulating cocktail) with anti-IL-1β blocking antibody or IL-1 receptor antagonist. The mCx-I activity was determined by using spectrophotometric measurements of the mitochondrial NADH-cytochrome c reductase activity. A: IB3-1 cells were treated with anti-IL-1β blocking antibody (30 ng/ml) or IL-1 receptor antagonist (10 ng/ml). The Figure shows the activity expressed as percentage (%), considering the average activity of S9 cells as 100%. Ab-Ctrl: anti-JNK2 monoclonal antibody as negative control (30 ng/ml). B: Spectrophotometric measurements of mitochondrial NADH-cytochrome c reductase activity of IB3-1 cells treated with increasing concentrations of anti-IL-1β blocking antibody (0, 1, 5, 15 and 30 ng/ml). C: Spectrophotometric measurements of mitochondrial NADH-cytochrome c reductase activity of IB3-1 cells treated with increasing concentrations of IL-1 receptor antagonist (0, 1, 5, 10 and 50 ng/ml). Measurements were performed in triplicate and data were expressed as mean ± SE of three independent experiments (n = 3). *indicates p<0.05 compared with basal IB3-1 cells.