A High Resolution Case Study of a Patient with Recurrent <i>Plasmodium vivax</i> Infections Shows That Relapses Were Caused by Meiotic Siblings

<div><p><i>Plasmodium vivax</i> infects a hundred million people annually and endangers 40% of the world's population. Unlike <i>Plasmodium falciparum</i>, <i>P. vivax</i> parasites can persist as a dormant stage in the liver, known as the hypnozoite, and these dormant forms can cause malaria relapses months or years after the initial mosquito bite. Here we analyze whole genome sequencing data from parasites in the blood of a patient who experienced consecutive <i>P. vivax</i> relapses over 33 months in a non-endemic country. By analyzing patterns of identity, read coverage, and the presence or absence of minor alleles in the initial polyclonal and subsequent monoclonal infections, we show that the parasites in the three infections are likely meiotic siblings. We infer that these siblings are descended from a single tetrad-like form that developed in the infecting mosquito midgut shortly after fertilization. In this natural cross we find the recombination rate for <i>P. vivax</i> to be 10 kb per centimorgan and we further observe areas of disequilibrium surrounding major drug resistance genes. Our data provide new strategies for studying multiclonal infections, which are common in all types of infectious diseases, and for distinguishing <i>P. vivax</i> relapses from reinfections in malaria endemic regions. This work provides a theoretical foundation for studies that aim to determine if new or existing drugs can provide a radical cure of <i>P. vivax</i> malaria.</p>