Histological analysis of lacrimal gland tissues.

<p>(A) Tissue sections of lacrimal glands from wild-type and <i>Itpr2^−/−</i>;<i>Itpr3^−/−</i> mice were stained by hematoxylin/eosin (HE) and observed under light microscopy. White arrowheads indicate inflammatory infiltrates. Scale bar: 50 µm. (B) Electron micrographs of lacrimal glands from wild-type and <i>Itpr2^−/−</i>; <i>Itpr3^−/−</i> mice. Scale bar: upper panels, 5 µm; lower panels, 2 µm. All experiments were performed at least three times, and representative data are shown. N; Nucleus, lu; lumen, ER; endoplasmic reticulum. (C) Relative lacrimal acinar cell area. The acinar cell area of wild-type (n = 54) and <i>Itpr2^−/−</i>;<i>Itpr3^−/−</i> (n = 59) lacrimal acinar cells was measured using HE-stained sections. Values represent the means ± SEM. Student’s t-test, ***, P<0.001.