Simulated FRAP-like experiment.

<p><b>A</b>: Recovery of relative fluorescence, , given by <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003629#pcbi.1003629.e506" target="_blank">Eq. (14)</a> (shown in red), obtained from the simulated <b>FRAP</b> experiment depicted in Video 0.3. For the initial conditions of the simulation (a totally bleached spherical volume of radius ) the half recovery time, , (<i>i.e.</i> the time at which ) is related to the diffusion coefficient by . From the simulation we obtain . Using we derive . <b>B</b>: Single molecule <b>MSD</b>, , computed using <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003629#pcbi.1003629.e461" target="_blank">Eq. (11)</a> for the bleached particles, as a function of time (shown in blue) and linear fit (shown in black). The slope of the fitting curve is which yields an estimated diffusion coefficient of .