Analysis of the pH dependence for the gelatinase activity present in the crude skin secretions of <i>L. labyrinthicus</i>.

<p>The gels (9% SDS-PAGE/gelatin) were incubated for 22 h under the following conditions: 10 mM CaCl₂, 50 mM sodium acetate, pH 4.0–5.0; 10 mM CaCl₂, 50 mM HEPES, pH 6.0–7.0; and 10 mM CaCl₂, 50 mM Tris-HCl, pH 8.0–10.0. The bands were quantified by densitometry and the values were normalized in relation to pH 10.0 gel. The values are shown close to their respective bands. Mw: molecular weight markers.