Downregulation of AKT phosphorylation is important for induction of dormant status.

<p>A) Immunoblot of the AKT/mTORC1 or ERK/p38 MAPK pathway in AsPC-1 cells cultured in hypoxia. B) Immunoblot of AKT signaling and HIF-1α in AsPC-1 cells expressing vector control, AKT-wt, or AKT-3A (inactive). C) Cell cycle status of the cells at day 7 in hypoxia. Percentages of the cells in S phase are indicated above the plot and in the right graph. D) ATP turnover measured by adding inhibitor cocktail for glycolysis and oxidative phosphorylation. N1, normoxia 1 day; H1, hypoxia 1 day; H7, hypoxia 7 days. E, F) Viable cell number (E) and percent cell death (F) of AsPC-1 cells cultured in hypoxia. *<i>p</i><0.05, **<i>p</i><0.01, ***<i>p</i><0.001.