Alternative splicing of PMCA in PMCA2- or PMCA3-deficient PC12 cells upon NFAT inhibition.

<p>Alternative splicing pattern at sites A and C of mRNA transcripts of PMCAs: <i>Atp21b1</i> (PMCA1) (<b>A</b>), <i>Atp21b2</i> (PMCA2) (<b>B</b>), <i>Atp21b3</i> (PMCA3) (<b>C</b>), <i>Atp21b4</i> (PMCA4) (<b>D</b>) was determined by RT-PCR in non-treated and 1 µM 11R-VIVIT-treated PC12 cells. RT-PCR product bands were quantified densitometrically, standardized to <i>Gapdh</i> and normalized to control cells, expressed as y = 1, both for non-treated (<b>E</b>) and 11R-VIVIT-treated cells (<b>F</b>). Student’s t-test was used for comparison of control cells with PMCA2- or PMCA3-reduced cells (n = 3). Bars represent mean values ± SEM. *P≤0.05. Symbols: control cells (C), PMCA2-deficient cells (_2), PMCA3-deficient cells (_3). Black arrows indicate the PCR product bands for PMCA2 site A and PMCA3 site C. White asterisks on the images of gels indicate the PCR product bands generated by alternative splicing that underwent a significant change upon NFAT inhibition with 11R-VIVIT.