The cardioprotective effect of NMN depends on Sirt1 expression.

<p>A, Acetylation levels of FoxO1 after 30 minutes of ischemia or sham operation with or without NMN administration. n = 4. n.s., not significant; # p<0.05, ## p<0.01 vs. respective sham-operated group. B and C, Either NMN (500 mg/kg) or vehicle (PBS) was administered (i.p. injection) to control or cardiac-specific Sirt1 knockout (Sirt1KO) mice 30 minutes before subjecting the mice to 30 minutes of ischemia followed by 24 hours of reperfusion (I/R). The extent of infarction was evaluated with TTC staining. Infarct area/AAR (B) and AAR (C) are shown. n = 4 to 5. n.s., not significant; * p<0.05, ## p<0.01 vs. respective control mice group. D, Schematic model of the protective effect of NMN against I/R injury. IPC: ischemic preconditioning; CR: caloric restriction; NMN: nicotinamide mononucleotide; NAD: nicotinamide adenine dinucleotide; I/R: ischemia/reperfusion.