<p><b>A</b>. miR-221 was predicted to regulate PTEN. <b>B</b>. After silencing miR-221, the mRNA level of PTEN was significantly up-regulated detected by real-time PCR. <b>C</b>. The corresponding change of PTEN in 231T-n and 231T-si221 cells was detected by western blot assay. *P<0.05; **P<0.01.</p
<p>A, shows the Western blot results of miRNA-21 target proteins, PDCD4 and PTEN in miRNA-21 over-ex...
<p>(a) The miR-BHRF1-3 target site in the PTEN 3’UTR according to a PAR-CLIP assay is shown (Skalsky...
<p>Quantitative real-time RT PCR was performed with RNA isolated from formalin fixed paraffin embedd...
<p>(A) Both QBC939 and HuCCT1 cells were transfected with PTEN siRNAs then treated with or without m...
<p>Cells were transfected with miR-21 inhibitor, inhibitor-negative control (NC) or blank control cu...
<p><b>A</b>. Several miRNAs were detected in 231 and 231T cells. miR-21 and miR-221 were statistical...
<p>A, Predicted miR-21 binding sites within the 3′-UTR of PTEN mRNA. The arrows display the mutation...
<p>(A) Construction of 3’UTR and mutated 3’UTR of PTEN into pMIR-REPORT vector. (B) In the cells tra...
<p>The cells in control, miR-21 mimics and miR-21 inhibitor were transfected with 100 nM scrambled o...
<p>(A) Ectopic expression of PTEN mRNA in MDA-MB-231/anti-miR-21 cells and MDA-MB-231/control cells ...
<p>Mmu-miR-141 expression (A) and PTEN mRNA level (B) were detected by real-time PCR in endometrial ...
<p>(A) Time course of pri- (small dotted line), pre- (large dotted line), or mature miR-21 (single u...
<p>A. Western blot analysis showed that PTEN protein expression decreased in IMR90 cells transfected...
<p>A. The PTEN expression at the mRNA (histogram) and protein level (representative Western blot ima...
<p>(A) <i>In vitro</i> pri-miR-21 processing with Drosha-expressing cell lysates, Drosha immunopreci...
<p>A, shows the Western blot results of miRNA-21 target proteins, PDCD4 and PTEN in miRNA-21 over-ex...
<p>(a) The miR-BHRF1-3 target site in the PTEN 3’UTR according to a PAR-CLIP assay is shown (Skalsky...
<p>Quantitative real-time RT PCR was performed with RNA isolated from formalin fixed paraffin embedd...
<p>(A) Both QBC939 and HuCCT1 cells were transfected with PTEN siRNAs then treated with or without m...
<p>Cells were transfected with miR-21 inhibitor, inhibitor-negative control (NC) or blank control cu...
<p><b>A</b>. Several miRNAs were detected in 231 and 231T cells. miR-21 and miR-221 were statistical...
<p>A, Predicted miR-21 binding sites within the 3′-UTR of PTEN mRNA. The arrows display the mutation...
<p>(A) Construction of 3’UTR and mutated 3’UTR of PTEN into pMIR-REPORT vector. (B) In the cells tra...
<p>The cells in control, miR-21 mimics and miR-21 inhibitor were transfected with 100 nM scrambled o...
<p>(A) Ectopic expression of PTEN mRNA in MDA-MB-231/anti-miR-21 cells and MDA-MB-231/control cells ...
<p>Mmu-miR-141 expression (A) and PTEN mRNA level (B) were detected by real-time PCR in endometrial ...
<p>(A) Time course of pri- (small dotted line), pre- (large dotted line), or mature miR-21 (single u...
<p>A. Western blot analysis showed that PTEN protein expression decreased in IMR90 cells transfected...
<p>A. The PTEN expression at the mRNA (histogram) and protein level (representative Western blot ima...
<p>(A) <i>In vitro</i> pri-miR-21 processing with Drosha-expressing cell lysates, Drosha immunopreci...
<p>A, shows the Western blot results of miRNA-21 target proteins, PDCD4 and PTEN in miRNA-21 over-ex...
<p>(a) The miR-BHRF1-3 target site in the PTEN 3’UTR according to a PAR-CLIP assay is shown (Skalsky...
<p>Quantitative real-time RT PCR was performed with RNA isolated from formalin fixed paraffin embedd...