DHA suppresses inflammasome induced IL-1β production.

<p>LPS primed. (A) THP-1 cells and (B) BMDMs were stimulated with ATP. DHA (50 uM or 100 uM) added or not at the priming step. Supernatants were collected and an ELISA was performed to check IL-1β levels. (C) Western blot analysis of cell supernatants and lysates from LPS primed THP-1 cells treated with ATP in the presence or absence of DHA. The indicated samples were analyzed for mature IL-1β, pro- and mature caspase-1, ASC, NLRP3, and actin. (D) BMDMs were LPS primed and stimulated with the inflammasome inducers nigericin, Poly(dA:dT), and flagellin. DHA was added, or not, at the priming step. Supernatants were collected and IL-1β levels checked by ELISA. Results are representatives of a minimum of three independent experiments. * P<0.05, **P<0.001, ***P<0.0002, ****P<0.0001.