<i>w</i>Au shortens the lifespan of the flies and grows exponentially within the hosts.

<p>(A) The survival of one hundred <i>Wolbachia</i>-free <i>iso</i>, <i>w</i>Au and <i>w</i>MelCS_b carrying male flies was checked every five days. The experiment was repeated once with comparable results and analysis was performed on both repetitions (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0099025#pone.0099025.s001" target="_blank">Figure S1E</a>). (B) The survival of one hundred males derived from <i>iso</i>, <i>w</i>Au and <i>w</i>MelCS_b tetracycline-treated stocks was checked every five days. The experiment was repeated once with comparable results and analysis was performed on both repetitions (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0099025#pone.0099025.s001" target="_blank">Figure S1F</a>). (C) qPCR on DNA isolated from males of <i>w</i>MelCS_b and <i>w</i>Au lines, collected every 10 days. Day 0 corresponds to 3–6 days-old flies, after day 40 the <i>w</i>Au carrying flies were not collected due to the high mortality. Each point represents a sample (each sample consisted of ten males), and lines are medians of the samples. Relative amount of <i>Wolbachia</i> genomic DNA was calculated using host Rpl32 as a reference gene and all values are relative to median of samples of <i>w</i>MelCS_b at day 0.