Oocyte meiotic maturation and P₄ secretion by follicles at 16 h after stimulation with hCG and EGF.

<p>Follicles were cultured individually for 12 days in the presence of DHT, E₂, or vehicle only (control; C), and then were stimulated with hCG and EGF for 16 h. During this period, normal oocytes advanced from GV stage through GVBD to MII with exclusion of the first polar body. The percentage of oocytes at each stage was calculated for follicles cultured with DHT (A-C) or with E₂ (D-F).(G and H) P₄ secretion from follicles stimulated for 16 h with hCG and EGF. All values are expressed as the mean ± SEM (n = 20 per group). *<i>P</i><0.05 vs. control.