<p>Follicles were cultured individually for 12 days with DHT, E<sub>2</sub> or vehicle only (control), and then were stimulated with hCG and EGF. Spindle fibers were detected by immunofluorescence for α-tubulin (green), while DNA was stained with propidium iodide (red). Scale bar in (A), 20 µm. The scale bar is applicable to all panels.</p
<p>A. a, represents a normal spindle in porcine oocyte. b and c represent abnormal spindle. In b1, c...
(A, B) Spindles from Metaphase I-arrested (emb-30(RNAi)) oocytes (A) or unarrested oocytes (B) treat...
<p>(A) Western blot from control, <i>klp-15 or klp-16(RNAi)</i> worms probed with the KLP-16 antibod...
<p>Follicles were cultured individually for 12 days with the vehicle only (control) or 10<sup>−6</su...
<p>Spindles were stained with α-Tubulin antibody (green) and chromosomes were counterstained with pr...
<p>(A) Samples were collected after oocytes had been cultured for 0.,2, 8, 9.5 and 12 h, correspondi...
<p>Follicles were cultured individually for 12 days in the presence of DHT, E<sub>2</sub>, or vehicl...
<p>(A) Haematoxylin and eosin staining of ovarian sections grafted under the kidney capsules. Left p...
<p>(A) Fluorescent immunolocalization of Kif4 (grey) in control and knock-down (KD) oocytes. Graphic...
<p>Oocytes cultured in M2 medium for 8h (MI) and 12h (MII), spindle was stained with FITC-α-tubulin ...
BACKGROUND: To ascertain whether different hormonal treatment protocols could affect metaphase II (M...
<p>(<b>A</b>) Microphotographs in the left panel (1, 4, 7) show the oocytes under bright field, wher...
<p>(<b>A</b>) An example of GVDB and MII oocytes observed under Nikon optics (left) and after DNA st...
(A) IF imaging of oocyte spindles in the ZYG-9 AID strain in either control or tac-1(RNAi) condition...
<p>Oocytes were injected at the GV stage and thereafter matured for cultured for 23 h. The cells are...
<p>A. a, represents a normal spindle in porcine oocyte. b and c represent abnormal spindle. In b1, c...
(A, B) Spindles from Metaphase I-arrested (emb-30(RNAi)) oocytes (A) or unarrested oocytes (B) treat...
<p>(A) Western blot from control, <i>klp-15 or klp-16(RNAi)</i> worms probed with the KLP-16 antibod...
<p>Follicles were cultured individually for 12 days with the vehicle only (control) or 10<sup>−6</su...
<p>Spindles were stained with α-Tubulin antibody (green) and chromosomes were counterstained with pr...
<p>(A) Samples were collected after oocytes had been cultured for 0.,2, 8, 9.5 and 12 h, correspondi...
<p>Follicles were cultured individually for 12 days in the presence of DHT, E<sub>2</sub>, or vehicl...
<p>(A) Haematoxylin and eosin staining of ovarian sections grafted under the kidney capsules. Left p...
<p>(A) Fluorescent immunolocalization of Kif4 (grey) in control and knock-down (KD) oocytes. Graphic...
<p>Oocytes cultured in M2 medium for 8h (MI) and 12h (MII), spindle was stained with FITC-α-tubulin ...
BACKGROUND: To ascertain whether different hormonal treatment protocols could affect metaphase II (M...
<p>(<b>A</b>) Microphotographs in the left panel (1, 4, 7) show the oocytes under bright field, wher...
<p>(<b>A</b>) An example of GVDB and MII oocytes observed under Nikon optics (left) and after DNA st...
(A) IF imaging of oocyte spindles in the ZYG-9 AID strain in either control or tac-1(RNAi) condition...
<p>Oocytes were injected at the GV stage and thereafter matured for cultured for 23 h. The cells are...
<p>A. a, represents a normal spindle in porcine oocyte. b and c represent abnormal spindle. In b1, c...
(A, B) Spindles from Metaphase I-arrested (emb-30(RNAi)) oocytes (A) or unarrested oocytes (B) treat...
<p>(A) Western blot from control, <i>klp-15 or klp-16(RNAi)</i> worms probed with the KLP-16 antibod...