Fucoidan promotes antigen presentation and antigen-specific T cell proliferation <i>in vivo</i>.

<p>(A) C57BL/6 mice were injected with PBS, OVA or OVA + fucoidan for 24 hrs, and the expression levels of MHC class I and II on the gated Lineage⁻CD11c⁺ cDCs in splenocytes from these mice were analyzed. (B) Purified CD8 T cells from OT-I or CD4 T cells from OT-II mice were labeled with CFSE and transferred into CD45.1 congenic mice, and 24 hrs later, mice were injected with PBS, OVA or OVA + fucoidan. After 3 day treatment, splenocytes from these mice were stained for CD45.2 to identify the donor OT-I or OT-II cells and the proliferation of these cells was determined by CFSE dilution. All data are from analyses of 6 individual mice each group (2 mice per experiment, total 3 independent experiments).