Gliadin induces barrier dysfunction and proinflammatory cytokines in poly I:C-treated NOD-DQ8 mice.

<p>After 24h of the final gavage, sections of small intestine were mounted in Ussing chambers and tissue conductance (mS/cm2) and isotopic flux (% Hot/h/cm²) were measured. Each dot represents an individual mouse and is a mean of 2 independent tissues. Stats: N = 3-4 mice per group, Two way ANOVA and Bonferroni post-test, *P<0.05 (<b>A</b>). Analysis of IL-12p70, TNFα and IL-10 in serum samples from poly I:C or PBS-treated mice on a gluten-free diet (GFD) or gavaged with gliadin for 2 weeks. IL-12p70/IL-10 ratio was determined. Serum concentration of cytokines was determined by a cytometric bead array inflammation kit. The dotted line represents the limit of detection. Stats: N = 4-5 mice per group, Two way ANOVA and Bonferroni post-test, *P<0.05, **P<0.01 gliadin versus GFD mice; #P<0.05 poly I:C versus PBS in Gliadin-gavaged mice (<b>B</b>).