Selective p110α inhibitors have lesser effect on cytokine/chemokine production in NK cells.

<p>NK cells from C57BL/6 mice were purified from spleen and expanded for 7–8 days in IL-2. (A–B) The NK cells were pre-treated with vehicle (0.1% DMSO or 0.1% ethanol) or 1 µM indicated inhibitors (TGX-221 was 0.5 µM) for 15 min, followed by stimulation with plate-bound anti-NKG2D mAb in the absence (<b>A</b>) or presence (<b>B</b>) of IL-12 and IL-18 for 18 h. The supernatants were collected and analyzed by Multiplex assay. The data are expressed as the means ± SEM of four independent NK cell preparations that were stimulated and analyzed concurrently. Statistical analysis was performed with one-way ANOVA using Prism 6 (GraphPad Software, Inc.) to compare the differences between vehicle and each inhibitor-treated group. *, <i>p</i><0.05, **, <i>p</i><0.01, and ***, <i>p</i><0.001.