<p>(<b>A</b>) Kinetics of hMPV infection. BDCA-1<sup>+</sup> and BDCA-3<sup>+</sup> mDCs were exposed to hMPV at a MOI = 5 for 12, 24, or 40 hours. The percentage of live cells expressing anti-hMPV antibody was quantified using flow cytometry. Filled grey curve = uninfected DCs, solid line = hMPV-infected DCs,. Data is from one donor and is representative of three donors. (<b>B</b>) Comparison of hMPV infection rates in BDCA-1<b><sup>+</sup></b> vs. BDCA-3<b><sup>+</sup></b> mDCs (mean + SEM of three donors). (<b>C</b>) Comparison of infection rates by RSV vs. hMPV in each subset (mean + SEM of three donors).</p
<p>pDCs (red) and mDCs (blue) were exposed to infectious IAV, HI IAV or left untreated for 4 hours, ...
<p>Representative ddPCR plots with corresponding housekeeping gene (<i>RPP30</i>) as insets shown in...
<p>(A) Representative flow cytometry plots showing the gating strategy used to define CD11c<sup>+</s...
<p>DC subsets were isolated and incubated with hMPV or media for 40 hours. Cytokine and chemokine le...
<p>(<b>A</b>) BDCA-1<sup>+</sup> and (<b>B</b>) BDCA-3<sup>+</sup> mDCs were isolated and incubated ...
<p>IFN-α production was assessed by ELISA in the supernatant of (<b>A</b>) BDCA-1<b><sup>+</sup></b>...
<p>(<b>A</b>) mDCs were exposed to infectious IAV, HI IAV or untreated for 4 hr, washed and exposed ...
<p>(<b>A</b>) pDCs (red) and mDCs (blue) were continuously exposed to IAV for 1, 6 or 24 hr and the ...
Quantification of flow cytometry analysis on inflammatory cell recruitment following HMPV TN/94-49 a...
<p>BDCA-1<sup>+</sup> and BDCA-3<sup>+</sup> mDC were incubated with RSV, hMPV, UV-hMPV, or media fo...
<p><b>(A)</b> MDDCs were co-cultured with mitomycin-treated C91-PL cells, and the percentage of MDDC...
<p>A) A549 cells were infected with RSV or hMPV at an MOI of 0.1. At the indicated time points the v...
<p>(<b>A</b>) mDCs were exposed to infectious IAV, HI IAV or left untreated for 4 hr and washed, the...
<p>MDDCs, MDMs or monocytes from the same blood donor were obtained as described in the <a href="htt...
<p><b>A</b>) BDCA1<sup>+</sup> mDCs or <b>B</b>) BDCA3<sup>+</sup> mDCs were co-cultured overnight (...
<p>pDCs (red) and mDCs (blue) were exposed to infectious IAV, HI IAV or left untreated for 4 hours, ...
<p>Representative ddPCR plots with corresponding housekeeping gene (<i>RPP30</i>) as insets shown in...
<p>(A) Representative flow cytometry plots showing the gating strategy used to define CD11c<sup>+</s...
<p>DC subsets were isolated and incubated with hMPV or media for 40 hours. Cytokine and chemokine le...
<p>(<b>A</b>) BDCA-1<sup>+</sup> and (<b>B</b>) BDCA-3<sup>+</sup> mDCs were isolated and incubated ...
<p>IFN-α production was assessed by ELISA in the supernatant of (<b>A</b>) BDCA-1<b><sup>+</sup></b>...
<p>(<b>A</b>) mDCs were exposed to infectious IAV, HI IAV or untreated for 4 hr, washed and exposed ...
<p>(<b>A</b>) pDCs (red) and mDCs (blue) were continuously exposed to IAV for 1, 6 or 24 hr and the ...
Quantification of flow cytometry analysis on inflammatory cell recruitment following HMPV TN/94-49 a...
<p>BDCA-1<sup>+</sup> and BDCA-3<sup>+</sup> mDC were incubated with RSV, hMPV, UV-hMPV, or media fo...
<p><b>(A)</b> MDDCs were co-cultured with mitomycin-treated C91-PL cells, and the percentage of MDDC...
<p>A) A549 cells were infected with RSV or hMPV at an MOI of 0.1. At the indicated time points the v...
<p>(<b>A</b>) mDCs were exposed to infectious IAV, HI IAV or left untreated for 4 hr and washed, the...
<p>MDDCs, MDMs or monocytes from the same blood donor were obtained as described in the <a href="htt...
<p><b>A</b>) BDCA1<sup>+</sup> mDCs or <b>B</b>) BDCA3<sup>+</sup> mDCs were co-cultured overnight (...
<p>pDCs (red) and mDCs (blue) were exposed to infectious IAV, HI IAV or left untreated for 4 hours, ...
<p>Representative ddPCR plots with corresponding housekeeping gene (<i>RPP30</i>) as insets shown in...
<p>(A) Representative flow cytometry plots showing the gating strategy used to define CD11c<sup>+</s...