Dose-dependent estrogen on osteogenic markers of osteo-adipogenic transdifferentiation of BMMSCs derived osteoblasts.

<p>After 14 days’ osteogenesis, different concentrations of 17beta-estradiol were added in adipogenic medium for 14 days and 14 days’ osteogenesis of BMMSCs in OVX group was used as positive control. O14∶14 days’ osteogenesis; O14+A7∶7 days’ adipogenesis after 14 days’ osteogenesis; O14+A14∶14 days’ adipogenesis after 14 days’ osteogenesis; E₂10⁻⁸ M: 14 days’ adipogenesis accompanied with 10⁻⁸ M of 17beta-estradiol after 14 days’ osteogenesis; E₂5×10⁻⁸ M: 14 days’ adipogenesis accompanied with 5×10⁻⁸ M of 17beta-estradiol after 14 days’ osteogenesis; E₂10⁻⁷ M: 14 days’ adipogenesis accompanied with 10⁻⁷ M of 17beta-estradiol after 14 days’ osteogenesis; E₂5×10⁻⁷ M: 14 days’ adipogenesis accompanied with 5×10⁻⁷ M of 17beta-estradiol after 14 days’ osteogenesis. A: ALP staining of osteo-adipogenic transdifferentiation of BMMSCs. B: ALP activity of osteo-adipogenic transdifferentiation of BMMSCs. The control value for ALP activity was 0.782±0.019 unit/mg protein. C: Alizarin red staining of osteo-adipogenic transdifferentiation of BMMSCs. D: The mineralization of osteo-adipogenic transdifferentiation of BMMSCs. The control value for mineralization was 0.985±0.020 OD. Data are represented as mean±SD of 3 independent experiments. *P<0.05 and **P<0.01.