RIG-I-enhanced type I IFN-independent apoptosis in response to JUNV infection.

<p><b>A–B.</b> shRNA-mediated stable down-regulation of RIG-I expression diminished DNA fragmentation in infected A549 cells. <b>A.</b> Efficient knock-down of RIG-I expression in RIG-I KD cells. Following poly(I:C) transfection RIG-I deficient (RIG-I KD) or competent (Control KD) cells were lysed and subjected to western blotting analysis. <b>B–D.</b> Cells were infected (MOI = 1.75 PFU/cell) with Candid#1 or Romero JUNV or mock-infected (Mock). <b>B.</b> Diminished DNA fragmentation in infected RIG-I-KD versus that of Control KD cells. <b>C.</b> Diminished DNA fragmentation in infected RIG-I-deficient Huh7.5 cells versus that of RIG-I competent Huh7. <b>D.</b> Induction of DNA fragmentation in type I IFN-deficient Vero and Vero E6 cells upon Candid#1 or Romero infection, respectively. <b>B–C.</b> Accumulation of mono- and oligonucleosomes in the cytoplasm was assayed by ELISA. Data represent the average of 3 replicates ±SEM. *** - P. value<0.001.