Zebrafish Rspo3 regulates dorsoventral patterning.

<p>(<b>A</b>) Expression patterns of the indicated marker genes in wild-type (WT) embryos or embryos injected with 4 ng control MO (cMO), 4 ng <i>rspo3</i> targeting MO1 or 600 pg <i>rspo3</i> mRNA, respectively. Embryos were analyzed at the shield stage by <i>in situ</i> hybridization using the indicated probes. Panels a–d and i-p are top views with animal pole up and dorsal to the right; panels e–h are lateral and dorsal views with animal pole up. Arrows indicate the width of the expression domains; asterisks indicate the edges of the expression domains. Percentages of embryos in each category were calculated and are shown in <b>B</b> (<i>chd</i>), <b>C</b> (<i>gsc</i>), <b>D</b><i>(eve1)</i> and <b>E</b> (<i>ved</i>). The total embryo numbers from three independent experiments are shown on the top of each bar. Scale bar = 200 µm.