Nuclear factor (NF)-κB DNA-binding activity, protein expression of the nuclear NF-κB p65 subunit and cytosolic inhibitory factor κBα (IκBα) in PC-3 cells incubated with CM from M2-type macrophages in the presence of EPA, DHA, or GW9662.

<p>The NF-κB DNA-binding activity was analyzed by a transcription factor ELISA (A). Protein levels of the IκBα subunit (B) and NF-κB p65 (C) were determined by Western blotting. Equal loading of proteins is illustrated by tubulin bands in the cytosolic fraction. Histone H1 expression is shown as an internal control in nuclear extracts. Data are presented as the mean ± SD, <i>n</i> = 3, and means with different letters significantly differ (<i>p</i><0.05).