Detection of virus in organs.

<p>Groups of 20 mice were infected with 2×10³ TCID₅₀ per animal of either KFDV P9605 or AHFV Zaki-1 via footpad inoculation. Two additional control groups of 5 mice each were inoculated with serum-free MEM. Five mice from each group were sacrificed at days 2, 4, 6, and 8 post-infection, and the controls groups were sacrificed at the end of study. Organs were harvested and homogenized as described in the Materials and Methods, and homogenates were titrated on BHK21 cells by limiting dilution. Results for the lung (A), brain (B), kidney (C), spleen (D), liver (E), and plasma (F) are shown. Each symbol represents one animal. Background cut-offs of 10¹ TCID₅₀ mg⁻¹ (lung in [A]), 10² TCID₅₀ mg⁻¹ (brain, kidney, spleen, and liver in [B–E]), and 10³ TCID₅₀ ml⁻¹ (plasma in [F]) were applied to organ and plasma titrations based on the control groups, and are shown as dashed lines on the lower axes of the respective graphs. Note in (F) that no animals were viremic. Each symbol represents one animal, and geometric means are indicated by horizontal black lines.