PPARγ expression in T cells and generation of CD4-PPARγ^KO mice.

<p>(A) Expression of PPARγ in FACS-purified CD4⁺, CD8⁺, and CD19⁺ cells from female wild type C57BL/6J mice was analyzed by quantitative real-time PCR and normalized to β-actin. Values represent the mean ± SEM, n = 4. **<i>P</i><0.01 when CD19 and CD8 were compared. (B) MACS-purified naïve (CD4⁺CD25⁻CD62L^highCD44^low) T cells from female wild type C57BL/6J mice were stimulated with plate-bound anti-CD3 and soluble anti-CD28 antibodies for the indicated times. PPARγ expression was determined by quantitative real-time PCR. Relative PPARγ expression level was calculated as the fold-change relative to 0 h. Values represent the mean ± SEM, n = 4. *<i>P</i><0.05, **<i>P</i><0.01 when 0 h, 24 h, and 48 h were compared. (C) FACS-sorted cells from female littermate control (Cre-) and CD4-PPARγ^KO (Cre+) mice were harvested and PPARγ expression was determined by quantitative real-time PCR. Values represent the mean ± SEM, n = 3. *<i>P</i><0.05. (D) Cre-mediated PPARγ deletion in cell lysate prepared from CD4⁺ T cells was confirmed by western blot analysis.