Characterization of primary cultures of first trimester trophoblast cells.

<p>Staining of primary cultures of isolated first trimester trophoblast cells. Top row, <b>s</b>taining with DAPI (blue) which labels cell nuclei, anti-cytokeratin-7 (CYT-7, red), anti-GPR54 (green) and the merge of staining with anti-cytokeratin-7 (red) and anti-GPR54 (green). Middle row, <b>s</b>taining with DAPI (blue), anti-vimentin (VIM, red), anti-GPR54 (green) and the merge of staining with anti-vimentin (red) and anti-GPR54 (green). Bottom row, staining with DAPI (blue), isotype matched mouse and rabbit IgG as negative controls and their merge. Scale bar indicates 200 µm.