Distribution of CAPS2 protein in the CAPS2^Δex3/Δex3 mouse cerebellum and cerebellar culture.

<p>(A, B) Sagittal sections of P7 wild-type (A) and CAPS2^Δex3/Δex3 (B) cerebellum were immunolabeled with anti-CAPS2 antibody. Immunosignal intensity of red dotted lines is shown by black lines in the graphs. Signal intensity from the other three images is shown in colored lines. Scale bars, 30 µm. (C, D) Sagittal sections of P21 wild-type (C) and CAPS2^Δex3/Δex3 (D) cerebellum were immunolabeled with an anti-CAPS2 antibody. E, external granular layer; M, molecular layer; P, Purkinje cell layer; I, internal granular layer. Scale bars, 20 µm. (E–M) Subcellular localization of CAPS2 (green; E, H, K) and calbindin (red; F, I, L) in wild-type (E–G), CAPS2^+/Δex3 (H–J) and CAPS2^Δex3/Δex3 (K–M) cerebellar primary cultures at 14 days <i>in vitro</i> (DIV). A merged image is shown in (G, J, M). Insets: higher magnification. P, Purkinje cell. Scale bars, 20 µm.