Interaction of Nei with DHU/G-substrate.

<p>(A) Changes in Trp fluorescence intensity during the interaction of Nei with DHU/G-substrate. Jagged traces display the experimental data whereas smooth curves correspond to the fit of the data to the kinetic model (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0100007#pone-0100007-g003" target="_blank">Fig. 3</a>). Concentrations of the DNA (µM) are shown next to the plots. (B) Time-course of appearance and disappearance of transient enzyme–substrate complexes during the cleavage of DHU/G by Nei. Modeling was done for 2 µM Nei and 2 µM substrate with the rate constants listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0100007#pone-0100007-t002" target="_blank">Table 2</a>. Accumulation of the reaction products detected by PAGE analysis (P^PAAG) is shown for comparison.