Representative Photomicrographs: Mouse kidneys were perfusion-fixed, removed, and stained with Flourojade-B, which stains early-necrotic cells bright green.

<p>Images taken of the cortico-medullary junction at 100× with no postprocessing other than pseudocolor simulating 532 nM flourescence. A)Female, wild-type vehicle control. B)Female, wild-type, estradiol-treated. C)Female, GPR30KO vehicle control. D)Female, GPR30KO, estradiol-treated. E)Male, wild-type vehicle control. F)Male, wild-type, estradiol-treated. G)Male, GPR30KO vehicle control. H)Male, GPR30KO, estradiol-treated. Second panel, wild-type vehicle vs. control. I)Wild-type, female vehicle control. J)Wild-type, female G1-treated.