siRNA inhibits ANDV replication in human primary lung endothelial cells (HMVEC-L).

<p>HMVEC-L were transfected with siRNAs for 6 h, and then infected with ANDV at MOI = 0.5. After 2 h of virus adsorption, the virus inoculum was removed and replaced with fresh media. (A) Viral protein levels as determined by Western blotting. Percent downregulation (%↓) of N or Gc protein represents percent decrease compared to non-targeting siRNA control. (B) Viral production as measured by immunofocus assays 48 h post-infection.