Adoptive transfer of NK cells does not alter pulmonary fibrosis in BIPF.

<p>(<b>A</b>) Recipient mice were injected with 1 million purified donor NK cells 12 h prior to bleomycin injection. (<b>B, C</b>) On day 21 leukocytes from BAL fluid, lungs, and spleen were collected, quantified, and stained with specific antibodies for leukocytes (CD45), NK cells (DX5), and T cells (TCRβ) and analyzed by flow cytometry. (<b>D, E</b>) Soluble collagen levels in BAL fluid and lung homogenates were measured by Sircol assay; total protein content was measured by BCA assay. Mean ± SEM of n = 5 mice per group; ** p<0.01 by <i>t</i>-test.