TFII-I depletion in human cells causes UV and cisplatin sensitivity.

<p>(A) Lysates of 293T cells transfected with the indicated siRNAs were blotted with indicated antibodies. (B) Quantitative PCR analysis of the Rev3L mRNA levels in mock or siRev3L transfected 293T cells. (C) Colony survival curves of 293T cells that were transfected with the indicated siRNAs and irradiated with increasing doses of UV. The mean and standard deviation (SD) of three independent experiments are shown. (D) Colony survival curves of 293T cells that were transfected with the indicated siRNAs and increasing doses of cisplatin. The mean and standard deviation (SD) of three independent experiments are shown. (E) Colony survival curves of U2OS cells that were transfected with the indicated siRNAs and irradiated with increasing doses of UV. The mean and standard deviation (SD) of three independent experiments are shown. (F) Colony survival curves of U2OS cells that were transfected with the indicated siRNAs and increasing doses of cisplatin. The mean and standard deviation (SD) of three independent experiments are shown.