Inhibition of Akt dephosphorylation is implicated in rapamycin-mediated neuroprotection in primary RGCs and the COH.

<p>Akt phosphorylation at sites Thr308 (A) and Ser473 (B) in primary RGCs was determined by western blotting. (C): The cell viability of primary RGCs was measured by MTT assay. (D): Akt phosphorylation at Thr308 in retinas was measured by western blotting. Values are presented as the mean ± SEM of 3 replicates, and experiments were repeated at least 2 times with similar results. **<i>p</i><0.01. Abbreviations: GLUT, glutamate; RAPA, rapamycin; Akti-1/2, an Akt inhibitor.