vCJD/BSE, sCJD and Alzheimer seeded Protein Misfolding Cyclic Amplification reactions using brain from transgenic mice as substrate.

<p>(<b>A–D</b>) WB PrP^res detection in PMCA reactions seeded with diluted 10% brain homogenates from human vCJD patient (vCJD Hu) or sporadic CJD (sCJD MM1, MV1, MV2, VV2 and VV1), ovine BSE (BSE Ov), porcine BSE (BSE po), or vCJD in primate (vCJD pri). A Scrapie isolate (WB control) and vCJD in human (vCJD Hu) isolate were used as positive control in an immunoblot (Sha31 anti PrP monoclonal antibody: epitope: YEDRYYRE, amino acid 145–152). Unseeded reactions (no seed) and reactions seeded with brain homogenate (10⁻² diluted, 10% - frontal cortex) from 3 Alzheimer affected patients (Alzh.) were included as specificity controls. The nature of the PMCA substrate and the number of amplification rounds are indicated.