Validation of overexpression of HIF-1α, TIMP1 and TFF3 in 10 pairs of gastric cancer vs. normal tissues.

<p>a and b, Detection of HIF-1α, TIMP1 and TFF3 mRNA expression in gastric cancer vs. normal tissues using PCR and qRT-PCR. Levels of HIF-1α, TIMP1, TFF3 mRNA were 2.55±0.56, 1.58±0.25, 2.16±0.59 folds up-regulated in tumor tissues, respectively compared to those of the normal ones. *p<0.01. c and d, Western blot analysis of HIF-1α protein. Tumor tissues expressed higher level of HIF-1α protein compared to the normal ones [p<0.01 (d). N, normal tissues; C, cancer tissues (c)].