Visual correspondence between ISODATA-derived and histology-derived non-viable tumor areas.

<p><b>A</b>) Representative T₂-weighted images of the hind limb region of two HIFU-treated mice before, 1 h after and 72 h after HIFU. The results of ISODATA segmentation with feature vector {T₁, T₂, ADC} are overlaid on the tumor pixels. NADH-diaphorase stained sections of tumors dissected at 72 h after HIFU were made at approximately the same location within the tumor and are shown at the bottom of each column. ROIs around the entire (black line) and non-viable (red line) tumor tissue were drawn manually. Data in the left column are from the animal presented in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0099936#pone-0099936-g001" target="_blank">Figure 1</a>. <b>B</b>) Similar data of a non-treated control mouse that was subjected to serial MRI measurements at the same time points (Day 0, Day 1 and Day 4) as the HIFU-treated animals. Scale bar = 1 mm.