Amplification of the <i>attB</i> and <i>attP</i> sequences generated after the three types of excision from PAISt.

<p>Products were detected by nested PCR using primer pairs described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0099345#pone-0099345-t002" target="_blank">Table 2</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0099345#pone-0099345-g002" target="_blank">Figure 2</a>. (<b>a</b>) PCR results resolved after electrophoresis in a 1% agarose gel. Lanes 1 to 3 show amplification of the <i>att</i> sites in the PAISt: <i>attL</i> (445 bp), <i>attI</i> (642 bp), and <i>attR</i> (341 bp). Lanes 4 to 6 show amplification of the <i>attP</i> sites in the circular structures (CS674, CS105 and CS569): recombination between <i>attR</i> and <i>attL</i> (<i>attP</i>-RxL, 380 bp), recombination between <i>attL</i> and <i>attI</i> (<i>att</i>P-LxI,663 bp), and recombination between <i>attR</i> and <i>attI</i> (<i>attP</i>-RxI, 359 bp). Lanes 7 to 9 shows amplification of the <i>attB</i> sites in the <i>S. turgidiscabies</i> chromosome: recombination between <i>attR</i> and <i>attL</i> (<i>attB</i>-RxL, 406 bp), recombination between <i>attL</i> and <i>attI</i> (<i>attB</i>-LxI, 424 bp), and recombination between <i>attR</i> and <i>attI</i> (<i>attB</i>-RxI, 624 bp). Lane M contains the 1 Kb ladder. (<b>b</b>) Sequences of the <i>att</i> sites with the palidromic sequences indicated in black. The flanking regions are presented in color to show recombination events within <i>att</i> sites (<i>attL</i> in green, <i>attI</i> in blue and <i>attR</i> in red).