Metabolite content in the diapausing pupal hemolymph during diapause termination resulting from injection of 20-Hydroxyecdysone (20E).

<p>Pupae were incubated at 25 °C after injection. The metabolites in the hemolymph were detected at 24, 48, and 72 h after injection. C represents the diapausing pupae not given the 20E injection and used as a control. (A) The PCA analysis for the metabolites in the hemolymph after injection. PC1 containing valine, leucine, proline, and phosphoric acid accounted for 39.1% of total variation, and PC2 containing butanoic acid, phenylalanine, tyrosine, and cholesterol accounted for 21.2% of total variation in brain PCA plot. The samples within a treatment group were circumscribed spatially by the dashed line. (B) Altered metabolite levels during diapause termination. The relative ratio is the ratio of the metabolite peak area to the internal standard (sucrose) peak area. All of the bars represent the mean ± S.D. from four replicates. *, <i>p</i><0.05; **, <i>p</i><0.01 (determined by one-way ANOVA).