Q-NPCs give rise to doublecortin-positive (DCX-positive) cells in vivo.

<p>Laser scanning images of <i>in vivo</i> differentiation and maturation of quadruple transgenic neuronal precursors (Q-NPCs) following transplantation into the lateral ventricle of newborn wild-type mice. (A, B) At 6 weeks after intraventricular transplantation, a portion of the transplanted cells (GFP-positive in green, additionally stained with an antibody against GFP) generated DCX-positive cells (red) but remained negative after staining (red) for glial marker GFAP (C), neuronal marker Tuj-1 (D) and oligodendrocyte marker O4 (E). DAPI staining is shown in blue. Scale bar (A, C–E) 50 µm, (B) 20 µm.