<i>Fusarium</i> species used to test specificity of an IGS sequence-based SYBR green or TaqMan qPCR assay designed to detect <i>Fusarium virguliforme</i>.

<p>Total DNA was extracted from pure cultures of each of these organisms and detection was assessed as cycle threshold (Ct) values generated with qPCR analysis.</p>a<p>Samples of all isolates were 1∶10 dilutions of a 1x DNA extract sample unless otherwise noted.</p>b<p>Samples were processed as described previously but SYBR green Master Mix was used for qPCR detection.